Submission ID 93751
| Poster Code | HR-P-10 |
|---|---|
| Title of Abstract | Myoblast transplantation, a potential universal treatment for muscular dystrophies |
| Abstract Submission | Duchenne Muscular Dystrophy (DMD) affects one in 3500 males and one in 50 million females. This disease is caused by a mutation in the dystrophin gene that is found on the X chromosome. The signs and symptoms are a weakening of the skeletal muscles, and after several years digestive, respiratory and cardiac difficulties leading to the death of the patient usually in their thirties. Since the cells are dystrophin-deficient, we are studying the possibility of treating the affected muscles by optimizing myoblast transplantation. This technique involves injecting healthy muscle cells into the patient's muscle to restore dystrophin, or other missing proteins, to muscle fibers that do not have any. We are very interested in healthy myoblast transplantation because it could be used to improve the quality of life of people with different muscular diseases, that are caused by a specific protein deficiency. The main limitation of this technique is the poor survival of the transplanted cells after injection. The experiment aimed to increase the survival rate of the transplanted cells. We pretreated the cells to be transplanted with Celastrol which is a neuroprotective anti-inflammatory. The results are a slight increase in the number of positive dystrophin fibers and a significant increase in the number of human nuclei indicating the survival of the transplanted cells. An optimized myoblast transplant protocol with Celastrol could therefore improve the quality of myoblast transplantation. |
| Please indicate who nominated you | FMED Université Laval |
| What Canadian Institutes of Health Research (CIHR) institute is your research most closely aligned? | Genetics Musculoskeletal Health and Arthritis Neurosciences, Mental Health and Addiction |
| What Canadian Institutes of Health Research (CIHR) pillar of health research does your research fall under? | Clinical |
| PDF of abstract | No file |
| Presenter and Author(s) | Camille Bouchard Catherine Gerard Jacques Tremblay |
