Submission ID 90384
| Poster Code | HR-P-87 |
|---|---|
| Title of Abstract | Developmental role of macrophages modelled with pluripotent stem cell derived intestinal tissue |
| Abstract Submission | Macrophages populate the embryo early in gestation but their function in the developmental process remains relatively unknown. Particularly, specification and function of macrophages in intestinal development remain unexplored. In order to study this event in human developmental context, we generated and combined intestinal organoid and macrophages from human pluripotent stem cells. Macrophages migrated into the organoid, proliferated, and occupied the emerging micro-anatomical niches of epithelial crypts and ganglia. They also acquired a transcriptomic profile alike fetal intestinal macrophages and displayed tissue resident macrophage behaviors, such as recruitment to injury site. Using this model, we show that macrophages reduce glycolysis in mesenchymal cells and limit tissue growth without affecting tissue architecture, in contrast to the pro-growth effect of enteric neurons. In conclusion, we engineered an intestinal tissue model populated with macrophages, and we suggest that resident macrophages contribute to regulation of metabolism and growth of the developing intestine. |
| Please indicate who nominated you | Department of Anatomy and Cell biology, Faculty of Medicine and Health Sciences, McGill university |
| What Canadian Institutes of Health Research (CIHR) institute is your research most closely aligned? | Human Development, Child and Youth Health Infection and Immunity |
| What Canadian Institutes of Health Research (CIHR) pillar of health research does your research fall under? | Biomedical |
| PDF of abstract | No file |
| Presenter and Author(s) | Gregor Andelfinger |
