Submission ID 131727
| Issue/Objective | Thanks to both medical and vector control efforts, elimination of gambiense Human African Trypanosomiasis (gHAT) as public health problem has been achieved in countries like Côte d'Ivoire and Burkina Faso. The World Health Organization (WHO) has set targets for interruption of transmission of gHAT by 2030. In this context, the performance of diagnostic algorithms for early detection of HAT reemergence remains to be assessed. |
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| Methodology/Approach | Our study represents a further step in the analysis on 8,648 dried blood spots collected during HAT rapid diagnostic tests (RDT) screening in gHAT historical foci in South West Burkina Faso and Centre West Côte d'Ivoire. We assessed the specificity of three molecular tests on 1,000 randomly selected samples from each country. We performed the Trypanozoon subgenus-specific m18S qPCR and RIME LAMP and the Trypanosoma brucei gambiense-specific TgsGP qPCR. |
| Results | No parasites were detected using parasitological investigations. The overall seroprevalence based on positivity to at least one RDT was 1.19% (103/8648, (0.83-1.55%). The specificities of m18S qPCR and TgsGP qPCR were 99% (990/1,000, 98.9-99.8%) and 99.8% (998/1,000, 95.5-100%) respectively. The RIME LAMP test was negative for all 1,000 specimens (specificity 100%). |
| Discussion/Conclusion | A previous study demonstrated low analytical sensitivities for qPCR m18S and qPCR TgsGP while that of the RIME LAMP was in the range of parasitaemias commonly observed in HAT patients. However, none of the three tests was entirely suitable for high-throughput use. To decide what is the best algorithm for HAT postelimination monitoring, data on costs for all possible diagnostics algorithms need to be considered. |
| Presenters and Affiliations | Charlie Franck Alfred Compaoré CIRDES/IRD Vincent Jamonneau Institut de Recherche pour le Développement INTERTRYP IRD-CIRAD Jacques Kaboré Centre International de recherche-développement sur l'élevage en zone subhumide, Université Nazi Boni, Sciences Biologiques Minayégninrin Koné Institut Pierre Richet de Bouaké Hamidou Ilboudo Institut de Recherche en Sciences de Recherche Clinique de Nanoro Mohamed Bamba Centre International de recherche-développement sur l'élevage en zone subhumide Hassane Sakandé Centre International de recherche-développement sur l'élevage en zone subhumide, Université Nazi Boni, Sciences Biologiques Dramane Kaba Institut Pierre Richet de Bouaké Adrien Marie Gaston Belem Université Nazi Boni, Sciences Biologiques Veerle Lejon Institut de Recherche pour le Développement INTERTRYP IRD-CIRAD |
